Journal: Antimicrobial Agents and Chemotherapy
Article Title: NDM-5-carried outer membrane vesicles impair the efficacy of antibiotics against bacterial infections
doi: 10.1128/aac.01805-24
Figure Lengend Snippet: N-OMVs invalidate MEM in G. mellonella to promote E. coli infection. ( A ) Illustration of the G. mellonella infection model. The bacteria and N-OMVs were injected into the left last ventral foot of the larvae (300 ± 50 mg), and MEM was injected into the opposite side 1 h later. Larvae survival rates ( n = 15) were recorded over 48 h. ( B ) Determination of the lethal dose of E. coli ATCC 25922 in G. mellonella . Larvae were injected with E. coli at 10 6 , 10 7 , and 10 8 CFU/mL, with the dose resulting in 80% mortality within 48 h selected for experiments. ( C ) Concentration screening of MEM. MEM at low dose (L-MEM, 5 mg/kg), medium dose (M-MEM, 10 mg/kg), and high dose (H-MEM, 15 mg/kg) were set to screen the drug dosage for the treatment group ( n = 15). ( D ) OMVs reduced the efficacy of MEM against E. coli . Larvae survival rates at 0, 12, 24, 36, and 48 h post-injection were monitored to assess the protective effect of N-OMVs on E. coli in vivo ( n = 15). Statistical significance was determined by the Kaplan-Meier test. ** P < 0.01, *** P < 0.001.
Article Snippet: To evaluate the ability of NDM-5-expressing E. coli -derived N-OMVs to protect E. coli ATCC 25922 against MEM killing, MIC and minimum bactericidal concentration (MBC) experiments were conducted.
Techniques: Infection, Bacteria, Injection, Concentration Assay, In Vivo